Evaluación del contenido de Withaferina A en Withania somnifera utilizando RMN y HPLC: una evaluación comparativa de los métodos de extracción tradicionales y PCSL

produced using Innovative Pressurised Cyclic Solid-Liquid Extraction, a technique increasingly adopted for its simplicity, speed, cost-effectiveness, and efficiency. The Withaferin A content was quantified by NMR, whose reliability was assessed through intra- and inter-day precision, as well as availability and stability tests. NMR proved to be a rapid and accurate method, confirming its suitability for determining active ingredient concentrations in natural extracts using an internal standard, with results consistent with HPLC © 2026 The Author(s). Chemistry & Biodiversity published by Wiley‐VHCA AG.