La validación transcriptómica integrada y experimental revela que la N-acetilcisteína mejora la insuficiencia hepática aguda mediante la regulación sinérgica multitarget de la piroptosis y las redes de señalización relacionadas

p62 levels, indicating restoration of autophagic flux. Compared with a pyroptosis inhibitor (Ac‑YVAD‑CMK), NAC showed superior efficacy in reversing TLR4 upregulation and cell‑cycle‑related protein abnormalities. NAC exerts a comprehensive hepatoprotective effect that surpasses that of a simple pyroptosis inhibitor by synergistically modulating pyroptosis, apoptosis, cell cycle, inflammatory recognition, and endocytosis pathways through multiple targets. These findings provide a theoretical foundation for expanding the clinical © 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany,