La L-Arginina atenúa el daño oxidativo y la apoptosis inducidos por estrés térmico en neutrófilos bovinos a través de la eliminación de ROS mediada por NFE2L2

bovine PMN, we first demonstrated that heat stress blocks nuclear factor erythroid 2-related factor 2 (NFE2L2) nuclear translocation; inhibition of NFE2L2 (ML385) abolished L-Arg's protection, verifying the NFE2L2-dependent ROS scavenging pathway. In conclusion, this study reveals that heat stress induces bovine PMN oxidative injury and apoptosis by suppressing NFE2L2-mediated ROS scavenging, and L-Arg restores PMN viability and immune resilience by reactivating the NFE2L2 antioxidant pathway. These findings provide a targeted L-Arg supplement strategy to improve PMN survival and disease resistance in