El extracto de orégano induce apoptosis e inhibe la autofagia en células HepG2 a través de la vía PI3K/AKT

pathway, and these effects were reversed by the ROS scavenger N-acetylcysteine (NAC), confirming ROS-dependent suppression of this pathway. Both network pharmacology and RNA-sequencing analyses further supported PI3K/AKT as a critical regulatory node. In summary, EAO exerts potent antitumor effects against hepatocellular carcinoma by inducing mitochondrial dysfunction and apoptosis, while concurrently suppressing autophagy through ROS-mediated inhibition of the PI3K/AKT pathway. These results highlight EAO as a promising Copyright © 2026 Xu, Zhu, Zhao, Li, Xu, Yang, Wang, Long, Zhang, Cao, Huang, Li,