Sensor electroquímico microscale ratiométrico para el monitoreo in situ de las fluctuaciones de ácido ascórbico inducidas por estrés oxidativo en células individuales

improve the detection accuracy, which achieved sensitive detection of AA with a detection limit of 0.79 μM. Notably, the ratiometric microsensor enabled the real-time monitoring of endogenous AA fluctuations in single PC12 cells under hydrogen peroxide stimulation. Furthermore, the microsensor confirmed that the supplementation of exogenous AA improved the intracellular AA level to defend against oxidative stress. The proposed strategy provided a simple and reliable platform for evaluating AA fluctuations at the single-cell level, holding promising prospects in the monitoring and treatment of neurological disorders.