La Withanolide A inhibe la agregación de hIAPP: Validación in silico, biofísica y en vivo basada en Drosophila

effect against hIAPP aggregation by increasing the lag phase, reducing β-sheet content, and inhibiting fibril formation of hIAPP. For in vivo validation using Drosophila model, Withanolide A was found to mitigate hIAPP oligomer-induced toxicity by reducing apoptosis, necrosis, and oxidative stress in the Drosophila gut, as confirmed by multiple cell death staining assays and reactive oxygen species (ROS) analysis. Besides, in diabetic flies, Withanolide A lowered glucose levels, demonstrating anti-diabetic activity. Thus, this work, for the first time, suggests that Withanolide A may be a potential candidate for