Desarrollo y evaluación funcional de un crioprotector de amplio espectro para la preservación de probióticos de nueva generación

supplementation. Functional assessments demonstrated preservation of key bacterial metabolic activities, including efficiency in short-chain fatty acid production and polyphenol catabolism, comparable to fresh cultures. Lastly, mouse study confirmed that freeze-dried G. uro remains metabolically active in producing urolithins at comparable levels to fresh cultures. To our knowledge, this work presents the first broad-spectrum LM that effectively preserves the viability and metabolic activity of strict anaerobes, providing a scalable solution for the development of NGP-integrated functional foods and health